Genotoxic potentials of some selected heavy metals exposure on Clarias gariepinus (Burchell, 1822) and Oreochromis niloticus (Linnaeus, 1758) using RAPD-PCR technique

Fish health and the aquatic ecosystem are strongly interwoven interrelated. The receives a range of anthropogenic chemicals which have toxicological or lethal effects on animals. aim this study was to determine level induced mutation genomic stability different sub-lethal doses (25%, 50% 75% LC50) heavy metal [Nickel (Ni), Mercury (Hg), Lead (Pb)and Zinc (Zn)] in Clarias gariepinus (Burchell, 1822) andOreochromis niloticus (Linnaeus, 1758) using RAPD-PCR technology over period 21 days. Four highly polymorphic RAPD markers were evaluated isolated DNA from both exposed fishes control after exposure. Observation profiles C. revealed more damaging effect as sub increased with zinc (a + b = 44 bands) mercury 41 nickel 37 lead 35 than 34 32 31 for O. when compare groups. Although, template decreased doses, higher observed (GTSPb 26.32% GTSHg 18.42% GTSZn 10.53% GTSNi 2.63%) 5.41% 0.0% -10.81% -18.92%). results obtained showed differential variation genetic stabilty niloticus. These observable differences might be due physiological structure fish species evaluated. This also confirms that RAPD–PCR is useful tools detecting genotoxic metals organisms but low reproducibility results, it recommended should used along other molecular techniques genotoxicity studies.Keywords: Heavy metals, Induced, Genotoxicity, Fish, Technique